As a technique, plant tissue culture includes growing plants and plant parts in controlled aseptic conditions on a medium containing nutrients and growth factors (vitamins and plant hormones) in test tube or any other container. For different species of plants, different kind of composition of media is required. Thus tissue culture procedure utilizes a laboratory type facility. However, plants can be produced from any part of the plant, but apical meristem is used to produce true to the type plants through tissue culture, this process is called MICROPRPAGATION or MERICLONING.


Use of tissue culture technology has come up as a very potential tool, which offers great promise to:

  • Speed up the process of disease free vegetative multiplication of newly developed varieties in short span of time, to shrink the time of 8 to 10 years by conventional methods. By using one million plants every year total seed of potato can be replaced every 5 years. This would facilitate quick exploitation of new varieties before they become degenerated by diseases and pests.

  • Produce disease and pest free material of old, but high yielding and very well adapted varieties.

  • Provide disease and pest free clones, which is very convenient and economic to transport even to distant places with out the danger of introducing new pests and diseases along with the seed tuber.

  • Improve the capacity to preserve and store the germplasm and field longevity of clones.